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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAK1 Monoclonal antibody (67707-1-Ig)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAK1 Monoclonal antibody (67707-1-Ig)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using TAK1 Monoclonal antibody (67707-1-Ig)

    WB analysis of NIH/3T3 using 67707-1-Ig (same clone as 67707-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAK1 Monoclonal antibody (67707-1-Ig)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TAK1 Monoclonal antibody (67707-1-Ig)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TAK1 Monoclonal antibody (67707-1-Ig)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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TAK1 Monoclonal antibody, PBS Only

TAK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67707-1-PBS
Clone No.1A5B2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67707-1-PBS targets TAK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAK1 fusion protein Ag21286 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579 aa, 64 kDa
Observed molecular weight67-70 kDa
GenBank accession numberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_2882898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43318
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.