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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAP1 Monoclonal antibody, PBS Only

TAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8A7

Cat no : 68412-1-PBS

Synonyms

ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAP1 Monoclonal antibody (68412-1-Ig)

    WB analysis using 68412-1-Ig (same clone as 68412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68412-1-Ig (TAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TAP1 Monoclonal antibody (68412-1-Ig)

    IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TAP1 Monoclonal antibody (68412-1-Ig)

    IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TAP1 Monoclonal antibody (68412-1-Ig)

    IF Staining of HepG2 using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TAP1 antibody (68412-1-Ig, Clone: 1B8A7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68412-1-PBS targets TAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TAP1 fusion protein Ag21303 相同性解析による交差性が予測される生物種
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Calculated molecular weight 81 kDa
Observed molecular weight70 kDa
GenBank accession numberBC014081
Gene symbol TAP1
Gene ID (NCBI) 6890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.