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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAPBP Polyclonal antibody (30500-1-AP)

    WB analysis using 30500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30500-1-AP (TAPBP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAPBP Polyclonal antibody (30500-1-AP)

    IF Staining of HeLa using 30500-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TAPBP antibody (30500-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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TAPBP Polyclonal antibody

TAPBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30500-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NGS 17, NGS17, TAP associated protein, TAP binding protein, TAP binding protein (tapasin), TAPA, tapasin, TAPBP, TPN, TPSN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MDA-MB-231 cells, THP-1 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30500-1-AP targets TAPBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAPBP fusion protein Ag30885 相同性解析による交差性が予測される生物種
Full Name TAP binding protein (tapasin)
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_003190
Gene Symbol TAPBP
Gene ID (NCBI) 6892
RRIDAB_3086339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAPBP, also known as tapasin, is a V-C1 (variable-constant) immunoglobulin superfamily (IgSF) molecule. It is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (PMID: 11920573).

Protocols

Product Specific Protocols
WB protocol for TAPBP antibody 30500-1-APDownload protocol
IHC protocol for TAPBP antibody 30500-1-APDownload protocol
IF protocol for TAPBP antibody 30500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols