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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of A549 cells using 12892-1-AP

    A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

  • Western Blot (WB) analysis of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of HeLa using 12892-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of mouse brain using 12892-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of C6 using 12892-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IP experiment of HeLa using 12892-1-AP

    IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IP experiment of mouse brain using 12892-1-AP

    IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of mouse brain using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of rat brain using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of human gliomas using 12892-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of mouse brain using 12892-1-AP

    Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of mouse brain using 12892-1-AP

    Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of HeLa using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of HeLa using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of Neuro-2a using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of Neuro-2a using 12892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    FC experiment of HeLa using 12892-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA
Cat No. 12892-1-AP
Publications(247)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA

Synonyms

TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, HeLa cells, C6 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inrat brain tissue, human gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells, Neuro-2a cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, chicken, zebrafish, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa, 35 kDa
GenBank accession numberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2200505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

Authors - Anna-Leigh Brown
View Article
humanWB,IHC

Science

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

Authors - Haiyang Yu
  • KO Validated
View Article
human,mouseIF

Nature

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.

Authors - Thomas O Vogler
View Article
mouseWB,IF

Nature

Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.

Authors - Lindsay A Becker
View Article
humanIF

Nat Med

Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.

Authors - Payam Mohassel
View Article
humanWB

Nat Cell Biol

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition

Authors - Shan Lu
View Article