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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using TARS Polyclonal antibody (14773-1-AP)

    IP experiment of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TARS Polyclonal antibody (14773-1-AP)

    IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TARS Polyclonal antibody (14773-1-AP)

    IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of MCF-7 using 14773-1-AP (same clone as 14773-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of HeLa using 14773-1-AP (same clone as 14773-1-PBS)

    Immunofluorescent analysis of Hela cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TARS Polyclonal antibody (14773-1-AP)

    IF Staining of HepG2 using 14773-1-AP (same clone as 14773-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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TARS Polyclonal antibody, PBS Only

TARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14773-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14773-1-PBS targets TARS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARS fusion protein Ag6614 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase
Calculated molecular weight 83 kDa
Observed molecular weight 66-72 kDa
GenBank accession numberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2200654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26639
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.