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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of HepG2 using 15067-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of HeLa using 15067-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of mouse liver using 15067-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IP experiment of mouse liver using 15067-1-AP

    IP result of anti-TARS2 (IP:15067-1-AP, 4ug; Detection:15067-1-AP 1:600) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse heart using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse heart using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse liver using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse liver using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TARS2 Polyclonal antibody (15067-1-AP)

    IF Staining of HepG2 using 15067-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15067-1-AP (TARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

TARS2 Polyclonal antibody

TARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15067-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Threonyl-tRNA synthetase-like 1, Threonyl-tRNA synthetase, Threonine--tRNA ligase, mitochondrial, Threonine  tRNA ligase, TARSL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 550 ug/ml
20 μL, 550 ug/ml
150 μL, 550 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15067-1-AP targets TARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARS2 fusion protein Ag7074 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC000541
Gene Symbol TARS2
Gene ID (NCBI) 80222
RRIDAB_2200668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TARS2 antibody 15067-1-APDownload protocol
IHC protocol for TARS2 antibody 15067-1-APDownload protocol
IF protocol for TARS2 antibody 15067-1-APDownload protocol
IP protocol for TARS2 antibody 15067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IP

Mol Cell

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

Authors - Sung-Hoon Kim
  • KD Validated
View Article
humanWB

Bioengineered

Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis.

Authors - Hui Tian
  • KD Validated
View Article
mouseWB

Redox Biol

Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle

Authors - Iliana López-Soldado
View Article
humanIHC

Int J Biol Markers

Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation

Authors - Lida Suo
View Article