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  • Featured Product
  • KD/KO Validated

TAU Monoclonal antibody, PBS Only

TAU Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9A8

Cat no : 66499-1-PBS

Synonyms

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAU Monoclonal antibody (66499-1-Ig)

    WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAU Monoclonal antibody (66499-1-Ig)

    WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TAU Monoclonal antibody (66499-1-Ig)

    WB analysis of HeLa using 66499-1-Ig (same clone as 66499-1-PBS)

    WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TAU Monoclonal antibody (66499-1-Ig)

    IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TAU Monoclonal antibody (66499-1-Ig)

    IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAU Monoclonal antibody (66499-1-Ig)

    IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAU Monoclonal antibody (66499-1-Ig)

    IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66499-1-PBS targets TAU in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TAU fusion protein Ag21926 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000558
Gene symbol TAU
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.