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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D15 Polyclonal antibody (17252-1-AP)

    WB analysis using 17252-1-AP (same clone as 17252-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBC1D15 Polyclonal antibody (17252-1-AP)

    WB analysis using 17252-1-AP (same clone as 17252-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17252-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D15 Polyclonal antibody (17252-1-AP)

    IF Staining of HeLa using 17252-1-AP (same clone as 17252-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17252-1-PBS in a different storage buffer formulation.

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TBC1D15 Polyclonal antibody, PBS Only

TBC1D15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 17252-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17252-1-PBS targets TBC1D15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D15 fusion protein Ag10625 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 15
Calculated molecular weight 691 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC028352
Gene Symbol TBC1D15
Gene ID (NCBI) 64786
RRIDAB_2878370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC07
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.