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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBC1D17 Polyclonal antibody

TBC1D17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20482-1-AP

Synonyms

TBC1 domain family member 17, TBC1 domain family, member 17, TBC1D17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D17 Polyclonal antibody (20482-1-AP)

    WB analysis using 20482-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    WB analysis of mouse thymus using 20482-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TBC1D17 Polyclonal antibody (20482-1-AP)

    WB analysis of Jurkat using 20482-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TBC1D17 Polyclonal antibody (20482-1-AP)

    IP experiment of Jurkat using 20482-1-AP

    IP result of anti-TBC1D17 (IP:20482-1-AP, 4ug; Detection:20482-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human breast cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human breast cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of mouse testis using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of mouse testis using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human heart using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human heart using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human liver cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBC1D17 Polyclonal antibody (20482-1-AP)

    IHC staining of human liver cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TBC1D17 Polyclonal antibody (20482-1-AP)

    IF Staining of A549 using 20482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20482-1-AP (TBC1D17 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse thymus tissue, Jurkat cells, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20482-1-AP targets TBC1D17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D17 fusion protein Ag14358 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 17
Calculated molecular weight 648 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC003516
Gene symbol TBC1D17
Gene ID (NCBI) 79735
RRIDAB_11232420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBC1D17 antibody 20482-1-APDownload protocol
IHC protocol for TBC1D17 antibody 20482-1-APDownload protocol
IF protocol for TBC1D17 antibody 20482-1-APDownload protocol
IP protocol for TBC1D17 antibody 20482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Bull (Beijing)

Hepatitis B virus core protein as a Rab-GAP suppressor driving liver disease progression

Authors - Yu Su
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells