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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D21 Polyclonal antibody (17463-1-AP)

    WB analysis using 17463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17463-1-AP (TBC1D21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using TBC1D21 Polyclonal antibody (17463-1-AP)

    WB analysis of rat testis using 17463-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17463-1-AP (TBC1D21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TBC1D21 Polyclonal antibody (17463-1-AP)

    WB analysis of mouse testis using 17463-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17463-1-AP (TBC1D21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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TBC1D21 Polyclonal antibody

TBC1D21 Polyclonal Antibody for WB, ELISA
Cat No. 17463-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TBC1 domain family member 21, TBC1 domain family, member 21, TBC1D21

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17463-1-AP targets TBC1D21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D21 fusion protein Ag11301 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 21
Calculated molecular weight 336 aa, 39 kDa
GenBank accession numberBC033516
Gene Symbol TBC1D21
Gene ID (NCBI) 161514
RRIDAB_3085524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D21 genes (also known as male germ cell RAB GTPase-activating protein MGCRABGAP) are related to sterility, as determined through cDNA microarray testing of human testicular tissues exhibiting spermatogenic defects. TBC1D21 is a protein specifically expressed in the testes that exhibits specific localizations of elongating and elongated spermatids during mammalian spermiogenesis.

Protocols

Product Specific Protocols
WB protocol for TBC1D21 antibody 17463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols