Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
17002-1-AP targets TBC1D23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBC1D23 fusion protein Ag10697 相同性解析による交差性が予測される生物種 |
| Full Name | TBC1 domain family, member 23 |
| Calculated molecular weight | 699 aa, 78 kDa |
| Observed molecular weight | 70-78 kDa |
| GenBank accession number | BC010221 |
| Gene Symbol | TBC1D23 |
| Gene ID (NCBI) | 55773 |
| RRID | AB_2240206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBC1D23 antibody 17002-1-AP | Download protocol |
| IHC protocol for TBC1D23 antibody 17002-1-AP | Download protocol |
| IF protocol for TBC1D23 antibody 17002-1-AP | Download protocol |
| IP protocol for TBC1D23 antibody 17002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Homozygous Mutations in TBC1D23 Lead to a Non-degenerative Form of Pontocerebellar Hypoplasia. | ||
Am J Hum Genet Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development. | ||
Int J Cancer Identification of frequently mutated genes with relevance to nonsense mediated mRNA decay in the high microsatellite instability cancers. | ||
J Cell Mol Med TBC1 domain family member 23 interacts with Ras-related protein Rab-11A to promote poor prognosis of non-small-cell lung cancer via β1-integrin. |