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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D25 Monoclonal antibody (67574-1-Ig)

    WB analysis using 67574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using TBC1D25 Monoclonal antibody (67574-1-Ig)

    WB analysis using 67574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TBC1D25 Monoclonal antibody (67574-1-Ig)

    IF Staining of HEK-293 using 67574-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TBC1D25 Monoclonal antibody (67574-1-Ig)

    IF Staining of NIH/3T3 using 67574-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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TBC1D25 Monoclonal antibody

TBC1D25 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67574-1-Ig
Clone No.2C7H3
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TBC1 domain family, member 25, TBC1 domain family member 25, 2C7H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, Neuro-2a cells
Positive IF/ICC detected inNIH/3T3 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67574-1-Ig targets TBC1D25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TBC1D25 fusion protein Ag29470 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 25
Observed molecular weight 80 kDa
GenBank accession numberBC101817
Gene Symbol TBC1D25
Gene ID (NCBI) 4943
RRIDAB_2882787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ3MII6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBC1D25 antibody 67574-1-IgDownload protocol
IF protocol for TBC1D25 antibody 67574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC,WB,IF

In Vitro Cell Dev Biol Anim

TBC1 domain family member 25 protects against myocardial apoptosis and the proinflammatory response triggered by ischemia-reperfusion injury through suppression of the TAK1-JNK/p38 MAPK signaling cascade

Authors - Ziwen Liu
View Article