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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBC1D4 Polyclonal antibody

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20024-1-AP

Synonyms

TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (20024-1-AP)

    WB analysis using 20024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20024-1-AP (TBC1D4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse colon using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse colon using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of rat skeletal muscle using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse skeletal muscle using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    IF Staining of HeLa using 20024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (20024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    FC experiment of HeLa using 20024-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    FC experiment of Jurkat using 20024-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse colon tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20024-1-AP targets TBC1D4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 4
Calculated molecular weight 147 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_014832
Gene symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

Protocols

Product Specific Protocols
WB protocol for TBC1D4 antibody 20024-1-APDownload protocol
IHC protocol for TBC1D4 antibody 20024-1-APDownload protocol
IF protocol for TBC1D4 antibody 20024-1-APDownload protocol
FC protocol for TBC1D4 antibody 20024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols