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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBC1D4 Polyclonal antibody

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24667-1-AP

Synonyms

TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (24667-1-AP)

    WB analysis using 24667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (24667-1-AP)

    WB analysis using 24667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of mouse colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of mouse colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human heart using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human heart using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D4 Polyclonal antibody (24667-1-AP)

    IF Staining of HeLa using 24667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells
Positive IHC detected inmouse colon tissue, human colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24667-1-AP targets TBC1D4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D4 fusion protein Ag20327 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 4
Calculated molecular weight 1299 aa, 147 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC151239
Gene symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

Protocols

Product Specific Protocols
WB protocol for TBC1D4 antibody 24667-1-APDownload protocol
IHC protocol for TBC1D4 antibody 24667-1-APDownload protocol
IF protocol for TBC1D4 antibody 24667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols