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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBCB Polyclonal antibody

TBCB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15782-1-AP

Synonyms

CG22, CKAP1, CKAPI, TBCB, tubulin folding cofactor B, Tubulin specific chaperone B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCB Polyclonal antibody (15782-1-AP)

    WB analysis using 15782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15782-1-AP (TBCB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IP experiment of HeLa using 15782-1-AP

    IP result of anti-TBCB (IP:15782-1-AP, 3ug; Detection:15782-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IF Staining of HeLa using 15782-1-AP

    Immunofluorescent analysis of Hela cells, using TBCB antibody 15782-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IF Staining of HeLa using 15782-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBCB antibody (15782-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, NCI-H1299 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15782-1-AP targets TBCB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBCB fusion protein Ag8473 相同性解析による交差性が予測される生物種
Full Name tubulin folding cofactor B
Calculated molecular weight 244 aa, 27 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC005969
Gene symbol TBCB
Gene ID (NCBI) 1155
RRIDAB_2302986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBCB antibody 15782-1-APDownload protocol
IF protocol for TBCB antibody 15782-1-APDownload protocol
IP protocol for TBCB antibody 15782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Histochem Cell Biol

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.

Authors - Lukas Juergens
View Article