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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCC Monoclonal antibody (68371-1-Ig)

    WB analysis using 68371-1-Ig (same clone as 68371-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Calu-3 cells using TBCC Monoclonal antibody (68371-1-Ig)

    WB analysis of Calu-3 using 68371-1-Ig (same clone as 68371-1-PBS)

    Calu-3 cells were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCC Monoclonal antibody (68371-1-Ig)

    IF Staining of HeLa using 68371-1-Ig (same clone as 68371-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBCC antibody (68371-1-Ig, Clone: 2B7D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

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TBCC Monoclonal antibody, PBS Only

TBCC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68371-1-PBS
Clone No.2B7D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68371-1-PBS targets TBCC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TBCC fusion protein Ag8962 相同性解析による交差性が予測される生物種
Full Name tubulin folding cofactor C
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC020170
Gene Symbol TBCC
Gene ID (NCBI) 6903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15814
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).