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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBCD Recombinant antibody, PBS Only

TBCD Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230209H7

Cat no : 82911-1-PBS

Synonyms

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCD Recombinant antibody (82911-1-RR)

    WB analysis using 82911-1-RR (same clone as 82911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TBCD Recombinant antibody (82911-1-RR)

    IF Staining of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using TBCD Recombinant antibody (82911-1-RR)

    FC experiment of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82911-1-RR

    Affinity and Kinetic Characterization of 82911-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82911-1-PBS targets TBCD in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBCD fusion protein Ag6310 相同性解析による交差性が予測される生物種
Full Name tubulin folding cofactor D
Calculated molecular weight 133 kDa
Observed molecular weight130 kDa
GenBank accession numberBC003094
Gene symbol TBCD
Gene ID (NCBI) 6904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.