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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBK1 Monoclonal antibody (67211-1-Ig)

    WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using TBK1 Monoclonal antibody (67211-1-Ig)

    WB analysis of U2OS using 67211-1-Ig (same clone as 67211-1-PBS)

    U2OS (WT and TBK1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 67211-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated
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TBK1 Monoclonal antibody, PBS Only

TBK1 Monoclonal Antibody for WB, ELISA
Cat No. 67211-1-PBS
Clone No.2D7B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67211-1-PBS targets TBK1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TBK1 fusion protein Ag28894 相同性解析による交差性が予測される生物種
Full Name TANK-binding kinase 1
Observed molecular weight 84 kDa
GenBank accession numberBC034950
Gene Symbol TBK1
Gene ID (NCBI) 29110
RRIDAB_2882504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHD2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.