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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBL1X Monoclonal antibody, PBS Only

TBL1X Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9A1

Cat no : 66955-1-PBS

Synonyms

EBI, SMAP55, TBL1, TBL1X

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using TBL1X Monoclonal antibody (66955-1-Ig)

    WB analysis of NIH/3T3 using 66955-1-Ig (same clone as 66955-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66955-1-Ig (TBL1X antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBL1X Monoclonal antibody (66955-1-Ig)

    IHC staining of human breast cancer using 66955-1-Ig (same clone as 66955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBL1X Monoclonal antibody (66955-1-Ig)

    IHC staining of human breast cancer using 66955-1-Ig (same clone as 66955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBL1X Monoclonal antibody (66955-1-Ig)

    IHC staining of human breast cancer using 66955-1-Ig (same clone as 66955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBL1X Monoclonal antibody (66955-1-Ig)

    IHC staining of human breast cancer using 66955-1-Ig (same clone as 66955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TBL1X Monoclonal antibody (66955-1-Ig)

    IF Staining of HEK-293 using 66955-1-Ig (same clone as 66955-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TBL1X antibody (66955-1-Ig, Clone: 1A9A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66955-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66955-1-PBS targets TBL1X in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBL1X fusion protein Ag5291 相同性解析による交差性が予測される生物種
Full Name transducin (beta)-like 1X-linked
Calculated molecular weight 526 aa, 57 kDa
Observed molecular weight 57 kDa, 62 kDa
GenBank accession numberBC032708
Gene symbol TBL1X
Gene ID (NCBI) 6907
RRIDAB_2882278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.