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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of COLO 320 using 12488-1-AP (same clone as 12488-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse placenta tissue using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of mouse placenta using 12488-1-AP (same clone as 12488-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of COLO 320 using 12488-1-AP (same clone as 12488-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of mouse pancreas using 12488-1-AP (same clone as 12488-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of human placenta using 12488-1-AP (same clone as 12488-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of HeLa using 12488-1-AP (same clone as 12488-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of COLO 320 using 12488-1-AP (same clone as 12488-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of Jurkat using 12488-1-AP (same clone as 12488-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Polyclonal antibody (12488-1-AP)

    WB analysis of COLO 320 using 12488-1-AP (same clone as 12488-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using TBL2 Polyclonal antibody (12488-1-AP)

    IP experiment of COLO 320 using 12488-1-AP (same clone as 12488-1-PBS)

    IP result of anti-TBL2 (IP:12488-1-AP, 4ug; Detection:12488-1-AP 1:800) with COLO 320 cells lysate 2400ug. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TBL2 Polyclonal antibody (12488-1-AP)

    IHC staining of human pancreas cancer using 12488-1-AP (same clone as 12488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TBL2 Polyclonal antibody (12488-1-AP)

    IHC staining of human heart using 12488-1-AP (same clone as 12488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TBL2 Polyclonal antibody (12488-1-AP)

    IHC staining of human heart using 12488-1-AP (same clone as 12488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using TBL2 Polyclonal antibody (12488-1-AP)

    IHC staining of human pancreas using 12488-1-AP (same clone as 12488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using TBL2 Polyclonal antibody (12488-1-AP)

    IHC staining of human pancreas using 12488-1-AP (same clone as 12488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TBL2 Polyclonal antibody (12488-1-AP)

    IF Staining of HepG2 using 12488-1-AP (same clone as 12488-1-PBS)

    Immunofluorescent analysis of () fixed HepG2 cells using 12488-1-AP (TBL2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 12488-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12488-1-PBS targets TBL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBL2 fusion protein Ag3200 相同性解析による交差性が予測される生物種
Full Name transducin (beta)-like 2
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012938
Gene Symbol TBL2
Gene ID (NCBI) 26608
RRIDAB_2199923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4P3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.