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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBL3 Polyclonal antibody (31150-1-AP)

    WB analysis using 31150-1-AP (same clone as 31150-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31150-1-AP (TBL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31150-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBL3 Polyclonal antibody (31150-1-AP)

    IF Staining of A431 using 31150-1-AP (same clone as 31150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBL3 antibody (31150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31150-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBL3 Polyclonal antibody (31150-1-AP)

    IF Staining of HeLa using 31150-1-AP (same clone as 31150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBL3 antibody (31150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31150-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31150-1-PBS targets TBL3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBL3 fusion protein Ag34900 相同性解析による交差性が予測される生物種
Full Name transducin (beta)-like 3
Calculated molecular weight 89 kDa
Observed molecular weight89 kDa
GenBank accession numberBC010231
Gene Symbol TBL3
Gene ID (NCBI) 10607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ12788
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transducin (beta)-like 3 (TBL3) is a protein of 808 amino acids with an apparent molecular weight of 89 kilodaltons.In mice, Tbl3 is a newly recognized nucleolar protein with regulatory roles in ribosome biogenesis (doi: 10.5315/wjh.v3.i3.93). In zebrafish, Tbl3 plays a tissue-specific role in regulating cell cycle rate during development(PMID: 22659140).