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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBP Polyclonal antibody

TBP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22246-1-AP

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TBP Polyclonal antibody (22246-1-AP)

    WB analysis of Jurkat using 22246-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBP Polyclonal antibody (22246-1-AP)

    WB analysis of COLO 320 using 22246-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using TBP Polyclonal antibody (22246-1-AP)

    WB analysis of SKOV-3 using 22246-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBP Polyclonal antibody (22246-1-AP)

    WB analysis of HeLa using 22246-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using TBP Polyclonal antibody (22246-1-AP)

    WB analysis of mouse colon using 22246-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TBP Polyclonal antibody (22246-1-AP)

    IF Staining of MCF-7 using 22246-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TBP antibody 22246-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, HeLa cells, mouse colon tissue, SKOV-3 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22246-1-AP is TBP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBP fusion protein Ag17740 相同性解析による交差性が予測される生物種
Full Name TATA box binding protein
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession numberBC110341
Gene symbol TBP
Gene ID (NCBI) 6908
RRIDAB_2879046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.

Protocols

Product Specific Protocols
WB protocol for TBP antibody 22246-1-APDownload protocol
IF protocol for TBP antibody 22246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Breast Cancer

Isoforms of IDH in breast carcinoma: IDH2 as a potent prognostic factor associated with proliferation in estrogen-receptor positive cases.

Authors - Hiroyuki Minemura
View Article
humanWB

Mol Med Rep

Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.

Authors - Qingye Li
View Article