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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBP Monoclonal antibody, PBS Only

TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3B2

Cat no : 66166-1-PBS

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of pig liver using 66166-1-Ig (same clone as 66166-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66166-1-PBS targets TBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBP fusion protein Ag12383 相同性解析による交差性が予測される生物種
Full Name TATA box binding protein
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession numberBC110341
Gene symbol TBP
Gene ID (NCBI) 6908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).