Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse testis tissue, mouse stomach tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12258-1-AP targets TBPL1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBPL1 fusion protein Ag2880 相同性解析による交差性が予測される生物種 |
| Full Name | TBP-like 1 |
| Calculated molecular weight | 186 aa, 21 kDa |
| Observed molecular weight | 21 kDa, 25 kDa |
| GenBank accession number | BC017559 |
| Gene Symbol | TBPL1 |
| Gene ID (NCBI) | 9519 |
| RRID | AB_10642841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62380 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| IHC protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| IP protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis | ||
Pharmacol Res Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway | ||
Cell Signal C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1. |