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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10177-2-AP

Synonyms

Protein TBRG4, KIAA0948, FASTKD4, FAST kinase domain-containing protein 4, CPR2

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using TBRG4 Polyclonal antibody (10177-2-AP)

    WB analysis of A431 using 10177-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10177-2-AP (TBRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TBRG4 Polyclonal antibody (10177-2-AP)

    WB analysis of HepG2 using 10177-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10177-2-AP (TBRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TBRG4 Polyclonal antibody (10177-2-AP)

    IHC staining of human ovary cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TBRG4 Polyclonal antibody (10177-2-AP)

    IHC staining of human stomach cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TBRG4 Polyclonal antibody (10177-2-AP)

    IHC staining of human stomach cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TBRG4 Polyclonal antibody (10177-2-AP)

    IF Staining of HepG2 using 10177-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TBRG4 antibody (10177-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10177-2-AP targets TBRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBRG4 fusion protein Ag0212 相同性解析による交差性が予測される生物種
Full Name transforming growth factor beta regulator 4
Calculated molecular weight 71 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC002732
Gene symbol TBRG4
Gene ID (NCBI) 9238
RRIDAB_2255699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBRG4 antibody 10177-2-APDownload protocol
IHC protocol for TBRG4 antibody 10177-2-APDownload protocol
IF protocol for TBRG4 antibody 10177-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols