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  • Featured Product
  • KD/KO Validated

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP

Conjugate

Unconjugated

Cat no : 16245-1-AP

Synonyms

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of HepG2 using 16245-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of human liver using 16245-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of mouse ovary using 16245-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of HeLa using 16245-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IP experiment of HepG2 using 16245-1-AP

    IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    IHC staining of human liver cancer using 16245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    IHC staining of human liver cancer using 16245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of U2OS cells using 16245-1-AP

    IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

  • Immunofluorescence (IF) / fluorescent staining of COS7 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of COS7 cells using 16245-1-AP

    IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of HeLa using 16245-1-AP

    Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : N/A
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16245-1-AP targets TBRG4 in WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBRG4 fusion protein Ag9176 相同性解析による交差性が予測される生物種
Full Name transforming growth factor beta regulator 4
Calculated molecular weight 631 aa, 71 kDa
Observed molecular weight 60 kDa, 71 kDa
GenBank accession numberBC017235
Gene symbol TBRG4
Gene ID (NCBI) 9238
RRIDAB_2271487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Protocols

Product Specific Protocols
WB protocol for TBRG4 antibody 16245-1-APDownload protocol
IHC protocol for TBRG4 antibody 16245-1-APDownload protocol
IF protocol for TBRG4 antibody 16245-1-APDownload protocol
IP protocol for TBRG4 antibody 16245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,HITS-CLIP

PLoS Genet

The FASTK family proteins fine-tune mitochondrial RNA processing

Authors - Akira Ohkubo
  • KO Validated
View Article
humanWB,IHC,IF,CoIP

BMC Cancer

High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma

Authors - Shanchun Tao
View Article
humanWB

Life Sci Alliance

Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.

Authors - Claudio Asencio
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)