Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 16245-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16245-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 16245-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16245-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16245-1-AP
IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.
IHC staining of human liver cancer using 16245-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16245-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS cells using 16245-1-AP
IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.
IF Staining of COS7 cells using 16245-1-AP
IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.
IF Staining of HeLa using 16245-1-AP
Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16245-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP

Synonyms

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells, Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : N/A
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

16245-1-AP targets TBRG4 in WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TBRG4 fusion protein Ag9176 相同性解析による交差性が予測される生物種
Full Name	transforming growth factor beta regulator 4
Calculated molecular weight	631 aa, 71 kDa
Observed molecular weight	60 kDa, 71 kDa
GenBank accession number	BC017235
Gene Symbol	TBRG4
Gene ID (NCBI)	9238
RRID	AB_2271487
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969Z0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Protocols

Product Specific Protocols
WB protocol for TBRG4 antibody 16245-1-AP	Download protocol
IHC protocol for TBRG4 antibody 16245-1-AP	Download protocol
IF protocol for TBRG4 antibody 16245-1-AP	Download protocol
IP protocol for TBRG4 antibody 16245-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,HITS-CLIP	PLoS Genet The FASTK family proteins fine-tune mitochondrial RNA processing Authors - Akira Ohkubo KO Validated View Article
human	WB,IHC,IF,CoIP	BMC Cancer High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma Authors - Shanchun Tao View Article
human	WB	Life Sci Alliance Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins. Authors - Claudio Asencio View Article
human	WB,IP	Nucleic Acids Res The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA Authors - Xuan Yang KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 16245-1-AP

WB analysis of human liver using 16245-1-AP

WB analysis of mouse ovary using 16245-1-AP

WB analysis of HeLa using 16245-1-AP

IP experiment of HepG2 using 16245-1-AP

IHC staining of human liver cancer using 16245-1-AP

IHC staining of human liver cancer using 16245-1-AP

IF Staining of U2OS cells using 16245-1-AP

IF Staining of COS7 cells using 16245-1-AP

IF Staining of HeLa using 16245-1-AP