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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

TBX2 Polyclonal antibody

TBX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16930-1-AP

Synonyms

T-box transcription factor TBX2, T-box protein 2, T-box 2, T box protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using TBX2 Polyclonal antibody (16930-1-AP)

    WB analysis of human placenta using 16930-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16930-1-AP (TBX2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TBX2 Polyclonal antibody (16930-1-AP)

    WB analysis of BxPC-3 using 16930-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16930-1-AP (TBX2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBX2 Polyclonal antibody (16930-1-AP)

    IHC staining of human kidney using 16930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBX2 Polyclonal antibody (16930-1-AP)

    IHC staining of human kidney using 16930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBX2 Polyclonal antibody (16930-1-AP)

    IF Staining of HeLa using 16930-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16930-1-AP (TBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, BxPC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16930-1-AP targets TBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBX2 fusion protein Ag10393 相同性解析による交差性が予測される生物種
Full Name T-box 2
Calculated molecular weight 702 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC052566
Gene symbol TBX2
Gene ID (NCBI) 6909
RRIDAB_2200526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBX2 antibody 16930-1-APDownload protocol
IHC protocol for TBX2 antibody 16930-1-APDownload protocol
IF protocol for TBX2 antibody 16930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Chem

Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins.

Authors - Bianca Del B Sahm
  • KD Validated
View Article
humanWB

Int J Mol Sci

Proteomics Analysis of the Polyomavirus DNA Replication Initiation Complex Reveals Novel Functional Phosphorylated Residues and Associated Proteins

Authors - Rama Dey-Rao
View Article