Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue, BxPC-3 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
16930-1-AP targets TBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBX2 fusion protein Ag10393 相同性解析による交差性が予測される生物種 |
| Full Name | T-box 2 |
| Calculated molecular weight | 702 aa, 74 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC052566 |
| Gene Symbol | TBX2 |
| Gene ID (NCBI) | 6909 |
| RRID | AB_2200526 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13207 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX2 antibody 16930-1-AP | Download protocol |
| IHC protocol for TBX2 antibody 16930-1-AP | Download protocol |
| IF protocol for TBX2 antibody 16930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Chem Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins.
| ||
Int J Mol Sci Proteomics Analysis of the Polyomavirus DNA Replication Initiation Complex Reveals Novel Functional Phosphorylated Residues and Associated Proteins |