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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBX20 Recombinant antibody

TBX20 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240385C12

Cat no : 83414-5-RR

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBX20 Recombinant antibody (83414-5-RR)

    WB analysis using 83414-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83414-5-RR (TBX20 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of k562 using TBX20 Recombinant antibody (83414-5-RR)

    FC experiment of k562 using 83414-5-RR

    1x10^6 k562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-5-RR, Clone:240385C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83414-5-RR

    Affinity and Kinetic Characterization of 83414-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-5-RR against Human TBX20 were performed. The affinity constant is 30 nM.

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Tested Applications

Positive WB detected inH9C2 cells, mouse heart tissue, rat heart tisue
Positive FC (Intra) detected ink562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83414-5-RR is TBX20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBX20 fusion protein Ag18501 相同性解析による交差性が予測される生物種
Full Name T-box 20
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC120946
Gene symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.

Protocols

Product Specific Protocols
WB protocol for TBX20 antibody 83414-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols