TBX21,T-bet Polyclonal antibody
TBX21,T-bet Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 13700-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NK-92 cells, A549 cells, mouse thymus tissue, RAW 264.7 cells |
| Positive IP detected in | mouse thymus tissue |
| Positive FC (Intra) detected in | NK92 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 18 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
Product Information
13700-1-AP targets TBX21,T-bet in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBX21,T-bet fusion protein Ag4618 相同性解析による交差性が予測される生物種 |
| Full Name | T-box 21 |
| Calculated molecular weight | 535 aa, 58 kDa |
| Observed molecular weight | 60-62 kDa |
| GenBank accession number | BC039739 |
| Gene symbol | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| RRID | AB_10666436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX21,T-bet antibody 13700-1-AP | Download protocol |
| IP protocol for TBX21,T-bet antibody 13700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.
| ||
Int Immunopharmacol Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway | ||
Int J Cardiol Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome. | ||
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
Biomed Pharmacother Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity. | ||
Front Pharmacol Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy. |
