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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TBX22 Polyclonal antibody (11133-1-AP)

    WB analysis of PC-3 using 11133-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11133-1-AP (TBX22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TBX22 Polyclonal antibody (11133-1-AP)

    WB analysis of mouse testis using 11133-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11133-1-AP (TBX22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TBX22 Polyclonal antibody (11133-1-AP)

    WB analysis of mouse testis using 11133-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11133-1-AP (TBX22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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TBX22 Polyclonal antibody

TBX22 Polyclonal Antibody for WB, ELISA
Cat No. 11133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CLPA, T box 22, T box protein 22, TBOX22, TBX22, TBXX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11133-1-AP targets TBX22 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBX22 fusion protein Ag1591 相同性解析による交差性が予測される生物種
Full Name T-box 22
Calculated molecular weight 58 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC014194
Gene Symbol TBX22
Gene ID (NCBI) 50945
RRIDAB_2200683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The frontonasal mass gives rise to the facial midline and fuses with the maxillary prominence to form the upper lip. T-box transcription factor 22 (Tbx22) belongs to the T-box family of transcription factors, which is essential for normal craniofacial development[PMID: 20033915]. Mutation in TBX22 in the frontnasal mass is reported in families with X-linked cleft palate and ankyloglossia [PMID:19816249].

Protocols

Product Specific Protocols
WB protocol for TBX22 antibody 11133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols