TBX4/5 Polyclonal antibody
TBX4/5 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 3 publications below |
Product Information
13178-1-AP targets TBX4/5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBX4/5 fusion protein Ag3845 相同性解析による交差性が予測される生物種 |
| Full Name | T-box 5 |
| Calculated molecular weight | 518 aa, 57 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC027942 |
| Gene symbol | TBX5 |
| Gene ID (NCBI) | 6910 |
| RRID | AB_10638435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX4/5 antibody 13178-1-AP | Download protocol |
| IF protocol for TBX4/5 antibody 13178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur Respir J Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes. | ||
Mol Biol Evol Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates. | ||
Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling. | ||
Stem Cell Reports Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures. | ||
iScience A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells. | ||
Sci Rep Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Young-Jae (Verified Customer) (12-06-2018) | Working good for western blot
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