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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBX6 Polyclonal antibody

TBX6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12447-1-AP

Synonyms

DFNB67, T box 6, T box protein 6, TBX6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of HeLa using 12447-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of mouse lung using 12447-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of A375 using 12447-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of human placenta using 12447-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of Raji using 12447-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of A549 using 12447-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of human lung using 12447-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using TBX6 Polyclonal antibody (12447-1-AP)

    IP experiment of human placenta using 12447-1-AP

    IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug.

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Tested Applications

Positive WB detected inHeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells
Positive IP detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBX6 fusion protein Ag3050 相同性解析による交差性が予測される生物種
Full Name T-box 6
Calculated molecular weight 436 aa, 47 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC026031
Gene symbol TBX6
Gene ID (NCBI) 6911
RRIDAB_2200836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.

Protocols

Product Specific Protocols
WB protocol for TBX6 antibody 12447-1-APDownload protocol
IP protocol for TBX6 antibody 12447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols