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TBXAS1 Monoclonal antibody

TBXAS1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F6G6

Cat no : 67371-1-Ig

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using TBXAS1 Monoclonal antibody (67371-1-Ig)

    WB analysis of human peripheral blood platelets using 67371-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TBXAS1 Monoclonal antibody (67371-1-Ig)

    WB analysis of A549 using 67371-1-Ig

    WB result of TBXAS1 antibody (67371-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBXAS1 transfected A549 cells.

  • Western Blot (WB) analysis of A549 cells using TBXAS1 Monoclonal antibody (67371-1-Ig)

    WB analysis of A549 using 67371-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TBXAS1 Monoclonal antibody (67371-1-Ig)

    WB analysis of human placenta using 67371-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig blood using TBXAS1 Monoclonal antibody (67371-1-Ig)

    WB analysis of pig blood using 67371-1-Ig

    pig blood were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TBXAS1 Monoclonal antibody (67371-1-Ig)

    IHC staining of human lung cancer using 67371-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TBXAS1 Monoclonal antibody (67371-1-Ig)

    IHC staining of human lung cancer using 67371-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using TBXAS1 Monoclonal antibody (67371-1-Ig)

    FC experiment of A549 using 67371-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.8 ug TBXAS1 Monoclonal antibody (67371-1-Ig, Clone:1F6G6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman peripheral blood platelets, A549 cells, human placenta tissue, pig blood
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBXAS1 fusion protein Ag29177 相同性解析による交差性が予測される生物種
Full Name thromboxane A synthase 1 (platelet)
Calculated molecular weight 60 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC014117
Gene symbol TBXAS1
Gene ID (NCBI) 6916
RRIDAB_2918487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).

Protocols

Product Specific Protocols
WB protocol for TBXAS1 antibody 67371-1-IgDownload protocol
IHC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
FC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Am J Physiol Renal Physiol

Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.

Authors - Katharina Mederle
View Article