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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCEAL1 Polyclonal antibody

TCEAL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11993-1-AP

Synonyms

p21, pp21, SIIR, TCEA like protein 1, TCEAL1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TCEAL1 Polyclonal antibody (11993-1-AP)

    WB analysis of mouse brain using 11993-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11993-1-AP (TCEAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TCEAL1 Polyclonal antibody (11993-1-AP)

    WB analysis of MCF-7 using 11993-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11993-1-AP (TCEAL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TCEAL1 Polyclonal antibody (11993-1-AP)

    WB analysis of MCF-7 using 11993-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11993-1-AP (TCEAL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TCEAL1 Polyclonal antibody (11993-1-AP)

    WB analysis of HeLa using 11993-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11993-1-AP (TCEAL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TCEAL1 Polyclonal antibody (11993-1-AP)

    WB analysis of mouse brain using 11993-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11993-1-AP (TCEAL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11993-1-AP targets TCEAL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEAL1 fusion protein Ag2653 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor A (SII)-like 1
Calculated molecular weight 159 aa, 21 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC000809
Gene symbol TCEAL1
Gene ID (NCBI) 9338
RRIDAB_2877814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCEAL1 is homologous to TFIIS, a conserved eukaryotic transcription elongation factor. The predicted 157-amino acid protein has a calculated molecular mass of 21 kD.On Western blots of mammalian cell extracts,it migrated as a 27-kD protein.

Protocols

Product Specific Protocols
WB protocol for TCEAL1 antibody 11993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols