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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCF20 Polyclonal antibody (32030-1-AP)

    WB analysis using 32030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32030-1-AP (TCF20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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TCF20 Polyclonal antibody

TCF20 Polyclonal Antibody for WB, ELISA
Cat No. 32030-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Stromelysin-1 PDGF-responsive element-binding protein, SPRE-binding protein, SPBP, Protein AR1, Nuclear factor SPBP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, U-251 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32030-1-AP targets TCF20 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCF20 fusion protein Ag36912 相同性解析による交差性が予測される生物種
Full Name transcription factor 20 (AR1)
Calculated molecular weight1960aa, 212 kDa
Observed molecular weight250 kDa
GenBank accession numberNM_005650
Gene Symbol TCF20
Gene ID (NCBI) 6942
RRIDAB_3670174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UGU0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription Factor 20 (TCF20) is a transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator (PMID: 10995766).

Protocols

Product Specific Protocols
WB protocol for TCF20 antibody 32030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols