Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, PC-3 cells, K-562 cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
21242-1-AP targets TCF3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TCF3 fusion protein Ag15734 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
| Calculated molecular weight | 654 aa, 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC110580 |
| Gene Symbol | TCF3 |
| Gene ID (NCBI) | 6929 |
| RRID | AB_10755295 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF3 antibody 21242-1-AP | Download protocol |
| IF protocol for TCF3 antibody 21242-1-AP | Download protocol |
| IP protocol for TCF3 antibody 21242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators | ||
J Cell Mol Med The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.
| ||
Mol Immunol Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development. | ||
Life Sci miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. | ||