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KD/KO Validated

TCF3 Polyclonal antibody

Name: TCF3 Polyclonal antibody
Brand: Proteintech
SKU: 21242-1-AP

TCF3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21242-1-AP

Synonyms

E2A, ITF1, Immunoglobulin enhancer-binding factor E12/E47, E47, Class B basic helix-loop-helix protein 21

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of Jurkat using 21242-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 21242-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21242-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21242-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 21242-1-AP
IP result of anti-TCF3 (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.
IF Staining of HeLa using 21242-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCF3 antibody (21242-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Cat No. 21242-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, PC-3 cells, K-562 cells, NIH/3T3 cells
Positive IP detected in	Jurkat cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

21242-1-AP targets TCF3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TCF3 fusion protein Ag15734 相同性解析による交差性が予測される生物種
Full Name	transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated molecular weight	654 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC110580
Gene symbol	TCF3
Gene ID (NCBI)	6929
RRID	AB_10755295
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TCF3 antibody 21242-1-AP	Download protocol
IF protocol for TCF3 antibody 21242-1-AP	Download protocol
IP protocol for TCF3 antibody 21242-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators Authors - Bofeng Liu View Article
human	WB,CoIP	Cell Signal Targeting SDCBP2 in acute myeloid leukemia Authors - Yan Du View Article
human	WB	J Cell Mol Med The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. Authors - Meiju Zhang KD Validated View Article
mouse	WB	Mol Immunol Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development. Authors - Wei Liang View Article
rat	IP	Life Sci miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Authors - Ting Liu View Article
mouse	WB	Nat Commun A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation Authors - Jianfeng Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCF3 Polyclonal antibody

TCF3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 21242-1-AP

WB analysis using 21242-1-AP

WB analysis of PC-3 using 21242-1-AP

WB analysis of K-562 using 21242-1-AP

IP experiment of Jurkat using 21242-1-AP

IF Staining of HeLa using 21242-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators

Targeting SDCBP2 in acute myeloid leukemia

The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.

Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.

miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.

A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation