Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, mouse brain tissue, JAR cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
14519-1-AP targets TCF7L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TCF7L1 fusion protein Ag5983 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 7-like 1 (T-cell specific, HMG-box) |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC058894 |
| Gene Symbol | TCF7L1 |
| Gene ID (NCBI) | 83439 |
| RRID | AB_2287033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCS4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| IHC protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| IF protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal | ||
Nat Commun Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling. | ||
Stem Cell Reports Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta. | ||
Oncogenesis TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer. | ||
iScience Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity | ||
Endocrinology Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility. |