Validation Data Gallery

Filter: AllWBIP

WB analysis of mouse liver using 13838-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13838-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF4/TCF7L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13838-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13838-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13838-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 13838-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 13838-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 13838-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13838-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 13838-1-AP
IP result of anti-TCF4/TCF7L2 (IP:13838-1-AP, 4ug; Detection:13838-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

TCF4/TCF7L2 Polyclonal antibody

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13838-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (26)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells
Positive IP detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 23 publications below
IHC	See 2 publications below
IF	See 3 publications below
CoIP	See 2 publications below

Product Information

13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TCF4/TCF7L2 fusion protein Ag4786 相同性解析による交差性が予測される生物種
Full Name	transcription factor 7-like 2 (T-cell specific, HMG-box)
Calculated molecular weight	619 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC032656
Gene Symbol	TCF4
Gene ID (NCBI)	6934
RRID	AB_2199812
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NQB0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TCF4/TCF7L2 antibody 13838-1-AP	Download protocol
IP protocol for TCF4/TCF7L2 antibody 13838-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal Authors - Jing Wang View Article
human	WB	Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer. Authors - Kai Li View Article
human	WB	Sci Rep TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis. Authors - Fengqin Dong View Article
human	WB,IF,CoIP	Front Cell Dev Biol Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin. Authors - Nanlan Yu View Article
human	WB	Mol Cancer Res Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition. Authors - Shuxuan Li View Article
human	WB	J Biol Chem HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis. Authors - Yifan Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 13838-1-AP

WB analysis using 13838-1-AP

WB analysis of human liver using 13838-1-AP

WB analysis of human brain using 13838-1-AP

WB analysis of human heart using 13838-1-AP

WB analysis of mouse heart using 13838-1-AP

WB analysis of mouse placenta using 13838-1-AP

WB analysis of rat liver using 13838-1-AP

WB analysis of mouse kidney using 13838-1-AP

IP experiment of mouse liver using 13838-1-AP

TCF4/TCF7L2 Polyclonal antibody

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin.

Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition.

HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis.