Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
25931-1-AP targets TCHP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TCHP fusion protein Ag23199 相同性解析による交差性が予測される生物種 |
| Full Name | trichoplein, keratin filament binding |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC004285 |
| Gene Symbol | TCHP |
| Gene ID (NCBI) | 84260 |
| RRID | AB_2880300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TCHP antibody 25931-1-AP | Download protocol |
| IHC protocol for TCHP antibody 25931-1-AP | Download protocol |
| IF protocol for TCHP antibody 25931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.
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Int J Mol Sci Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization
| ||
J Cell Physiol Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.
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Nat Commun Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function |