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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCN2 Polyclonal antibody, PBS Only

TCN2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12157-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis using 12157-1-AP (same clone as 12157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis of rat liver using 12157-1-AP (same clone as 12157-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of human pancreas cancer using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12157-1-PBS targets TCN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825 相同性解析による交差性が予測される生物種
Full Name transcobalamin II; macrocytic anemia
Calculated molecular weight 400 aa, 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011239
Gene symbol TCN2
Gene ID (NCBI) 6948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)