Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 11003-1-AP
WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.
WB analysis of HeLa using 11003-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 11003-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11003-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11003-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11003-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11003-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11003-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11003-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 11003-1-AP
Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of MCF-7 using 11003-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (11003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TCOF1 Polyclonal antibody

TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11003-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

treacle, MFD1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Neuro-2a cells, Jurkat cells
Positive IHC detected in	human colon cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

11003-1-AP targets TCOF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TCOF1 fusion protein Ag1447 相同性解析による交差性が予測される生物種
Full Name	Treacher Collins-Franceschetti syndrome 1
Calculated molecular weight	97 kDa, 144 kDa, 152 kDa
Observed molecular weight	220 kDa
GenBank accession number	BC014559
Gene Symbol	TCOF1
Gene ID (NCBI)	6949
RRID	AB_2202516
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13428
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TCOF1 antibody 11003-1-AP	Download protocol
IHC protocol for TCOF1 antibody 11003-1-AP	Download protocol
IF protocol for TCOF1 antibody 11003-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Cell-fate determination by ubiquitin-dependent regulation of translation. Authors - Achim Werner View Article
human		Nat Commun Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response. Authors - Stephen M Rawlinson View Article
human	WB, IF	Proc Natl Acad Sci U S A Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response. Authors - Alberto Ciccia KD Validated View Article
human	WB	Elife Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions. Authors - Achim Werner View Article
human	WB, IF	Int J Mol Sci TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth. Authors - Ahmed Hassan View Article
human	WB,IF	Cancer Sci TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC. Authors - Chaoshen Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11003-1-AP

WB analysis of HeLa using 11003-1-AP

WB analysis of Neuro-2a using 11003-1-AP

WB analysis of Jurkat using 11003-1-AP

WB analysis of HeLa using 11003-1-AP

IHC staining of human colon cancer using 11003-1-AP

IHC staining of human colon cancer using 11003-1-AP

IHC staining of human colon cancer using 11003-1-AP

IHC staining of human lymphoma using 11003-1-AP

IF Staining of MCF-7 using 11003-1-AP

IF Staining of MCF-7 using 11003-1-AP

TCOF1 Polyclonal antibody

TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cell-fate determination by ubiquitin-dependent regulation of translation.

Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.