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  • KD/KO Validated

TCOF1 Polyclonal antibody

TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11003-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

treacle, MFD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Neuro-2a cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11003-1-AP targets TCOF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCOF1 fusion protein Ag1447 相同性解析による交差性が予測される生物種
Full Name Treacher Collins-Franceschetti syndrome 1
Calculated molecular weight 97 kDa, 144 kDa, 152 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC014559
Gene Symbol TCOF1
Gene ID (NCBI) 6949
RRIDAB_2202516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCOF1 antibody 11003-1-APDownload protocol
IHC protocol for TCOF1 antibody 11003-1-APDownload protocol
IF protocol for TCOF1 antibody 11003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Cell-fate determination by ubiquitin-dependent regulation of translation.

Authors - Achim Werner
human

Nat Commun

Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.

Authors - Stephen M Rawlinson
humanWB, IF

Proc Natl Acad Sci U S A

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Authors - Alberto Ciccia
  • KD Validated
humanWB

Elife

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

Authors - Achim Werner
humanWB, IF

Int J Mol Sci

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Authors - Ahmed Hassan
humanWB,IF

Cancer Sci

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Authors - Chaoshen Wu
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