Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10320-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10320-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10320-1-AP
IP result of anti-TCP1 (IP:10320-1-AP, 4ug; Detection:10320-1-AP 1:300) with HeLa cells lysate 1600ug.
IHC staining of human colon cancer using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 10320-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 10320-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TCP1 antibody (10320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 10320-1-AP
Immunofluorescent analysis of MCF-7 cells using 10320-1-AP (TCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 10320-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug TCP1 Polyclonal antibody (10320-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

TCP1 Polyclonal antibody

TCP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10320-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CCT alpha, CCT1, CCTa, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse heart tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, NCI-H1299 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue, human colon tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10320-1-AP targets TCP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TCP1 fusion protein Ag0389 相同性解析による交差性が予測される生物種
Full Name	t-complex 1
Calculated molecular weight	60 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC000665
Gene Symbol	TCP1
Gene ID (NCBI)	6950
RRID	AB_10694136
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17987
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protocols

Product Specific Protocols
WB protocol for TCP1 antibody 10320-1-AP	Download protocol
IHC protocol for TCP1 antibody 10320-1-AP	Download protocol
IF protocol for TCP1 antibody 10320-1-AP	Download protocol
IP protocol for TCP1 antibody 10320-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mechanism of orphan subunit recognition during assembly quality control Authors - Yuichi Yagita View Article
human	WB	J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article
mouse,human	WB	Front Cell Dev Biol TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis Authors - Wen-Shuai Tang View Article
mouse	WB	J Alzheimers Dis Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid Authors - Ye Shan View Article
mouse	IP	Virology Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex. Authors - Sandhya Gopal View Article
mouse	WB	J Agric Food Chem A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells. Authors - Cheng Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10320-1-AP

WB analysis of mouse heart using 10320-1-AP

IP experiment of HeLa using 10320-1-AP

IHC staining of human colon cancer using 10320-1-AP

IHC staining of human colon using 10320-1-AP

IHC staining of human colon using 10320-1-AP

IHC staining of human liver cancer using 10320-1-AP

IHC staining of human liver cancer using 10320-1-AP

IHC staining of human colon cancer using 10320-1-AP

IHC staining of human colon cancer using 10320-1-AP

IF Staining of HeLa using 10320-1-AP

IF Staining of MCF-7 using 10320-1-AP

FC experiment of HeLa using 10320-1-AP

TCP1 Polyclonal antibody

TCP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mechanism of orphan subunit recognition during assembly quality control

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis

Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid

Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.