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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCP1 Monoclonal antibody

TCP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C2F6

Cat no : 68183-1-Ig

Synonyms

D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCP1 Monoclonal antibody (68183-1-Ig)

    WB analysis using 68183-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68183-1-Ig (TCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TCP1 Monoclonal antibody (68183-1-Ig)

    IF Staining of HeLa using 68183-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCP1 antibody (68183-1-Ig, Clone: 2C2F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TCP1 Monoclonal antibody (68183-1-Ig)

    FC experiment of HeLa using 68183-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TCP1 (68183-1-Ig, Clone:2C2F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68183-1-Ig targets TCP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TCP1 fusion protein Ag0389 相同性解析による交差性が予測される生物種
Full Name t-complex 1
Calculated molecular weight 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000665
Gene symbol TCP1
Gene ID (NCBI) 6950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCP1 antibody 68183-1-IgDownload protocol
IF protocol for TCP1 antibody 68183-1-IgDownload protocol
FC protocol for TCP1 antibody 68183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols