• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TCP11L2 Recombinant antibody (83227-1-RR)

    WB analysis using 83227-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDCK cells using TCP11L2 Recombinant antibody (83227-1-RR)

    WB analysis of MDCK using 83227-1-RR

    MDCK cells were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using TCP11L2 Recombinant antibody (83227-1-RR)

    IF Staining of U-251 using 83227-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of A431 cells using TCP11L2 Recombinant antibody (83227-1-RR)

    FC experiment of A431 using 83227-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using TCP11L2 Recombinant antibody (83227-1-RR)

    FC experiment of SH-SY5Y using 83227-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83227-1-RR

    Affinity and Kinetic Characterization of 83227-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TCP11L2 Recombinant antibody

TCP11L2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83227-1-RR
Clone No.240096F6

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MDCK cells, U251 cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inA431 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83227-1-RR targets TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCP11L2 fusion protein Ag11185 相同性解析による交差性が予測される生物種
Full Name t-complex 11 (mouse)-like 2
Calculated molecular weight 519 aa, 58 kDa
Observed molecular weight60 kDa
GenBank accession numberBC033617
Gene Symbol TCP11L2
Gene ID (NCBI) 255394
RRIDAB_3670910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N4U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)

Protocols

Product Specific Protocols
WB protocol for TCP11L2 antibody 83227-1-RRDownload protocol
IF protocol for TCP11L2 antibody 83227-1-RRDownload protocol
FC protocol for TCP11L2 antibody 83227-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols