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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCTN3 Polyclonal antibody (16085-1-AP)

    WB analysis using 16085-1-AP (same clone as 16085-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16085-1-AP (TCTN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16085-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Hela cells using TCTN3 Polyclonal antibody (16085-1-AP)

    IP experiment of Hela cells using 16085-1-AP (same clone as 16085-1-PBS)

    IP result of anti-TCTN3 (16085-1-AP for IP and Detection). This data was developed using the same antibody clone with 16085-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TCTN3 Polyclonal antibody (16085-1-AP)

    IHC staining of human lung using 16085-1-AP (same clone as 16085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16085-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TCTN3 Polyclonal antibody (16085-1-AP)

    IHC staining of human lung using 16085-1-AP (same clone as 16085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16085-1-PBS in a different storage buffer formulation.

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TCTN3 Polyclonal antibody, PBS Only

TCTN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16085-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

TECT3, TCTN 3, PSEC0041, OFD4, JBTS18

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16085-1-PBS targets TCTN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCTN3 fusion protein Ag9084 相同性解析による交差性が予測される生物種
Full Name tectonic family member 3
Calculated molecular weight 607 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009494
Gene Symbol TCTN3
Gene ID (NCBI) 26123
RRIDAB_10638442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NUS6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TCTN3 (tectonic-3 or TECT3) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. TCTN3 is necessary for transduction of the sonic hedgehog (SHH) signaling pathway. Orofaciodigital syndromes (OFDSs) consist of a group of heterogeneous disorders characterized by abnormalities in the oral cavity, face, and digits and associated phenotypic abnormalities. Recently, TCTN3 mutations are found as the cause of an extreme form of OFD associated with bone dysplasia, tibial defect, cystic kidneys, and brain anomalies.