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  • Featured Product
  • KD/KO Validated

TDP-43 Recombinant antibody, PBS Only (Detector)

TDP-43 Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

11N20

Cat no : 80001-1-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    WB analysis using 80001-1-RR (same clone as 80001-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80001-1-RR (TDP43 antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    WB analysis of HeLa using 80001-1-RR (same clone as 80001-1-PBS)

    WB result of TDP-43 antibody (80001-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HAP1 using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    WB analysis of HAP1 using 80001-1-RR (same clone as 80001-1-PBS)

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80001-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP80000-1

    Sandwich ELISA standard curve of MP80000-1

    Sandwich ELISA standard curve ofMP80000-1, TDP-43 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60019-2-PBS. Detection antibody: 80001-1-PBS. Standard: Ag1833. Range: 78.1-5000 pg/mL.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of rat brain using 80001-1-RR (same clone as 80001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 4x lens). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of spinal cord slides from ALS patients using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of spinal cord slides from ALS patients using 80001-1-RR (same clone as 80001-1-PBS)

    IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the spinal cord slides from ALS patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of frontal cortex from FTLD-TDP type B using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of frontal cortex from FTLD-TDP type B using 80001-1-RR (same clone as 80001-1-PBS)

    IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of human gliomas using 80001-1-RR (same clone as 80001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of human gliomas using 80001-1-RR (same clone as 80001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IHC staining of mouse brain using 80001-1-RR (same clone as 80001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IF Staining of rat brain using 80001-1-RR (same clone as 80001-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80001-1-RR (TDP-43 antibody) at dilution of 1:150 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HAP1 using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    IF Staining of HAP1 using 80001-1-RR (same clone as 80001-1-PBS)

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80001-1-RR at 1:400 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TDP-43 (N-terminal) Recombinant antibody (80001-1-RR)

    FC experiment of HeLa using 80001-1-RR (same clone as 80001-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (80001-1-RR, Clone:11N20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80001-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80001-1-PBS targets TDP-43 as part of a matched antibody pair:

MP80000-1: 60019-2-PBS capture and 80001-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).

80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.