• Featured Product
  • KD/KO Validated

TDP-43 Recombinant antibody, PBS Only (Detector)

TDP-43 Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

11N20

Cat no : 80001-1-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80001-1-PBS targets TDP-43 as part of a matched antibody pair:

MP80000-1: 60019-2-PBS capture and 80001-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TDP-43
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).

80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.