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  • Featured Product
  • KD/KO Validated

TDP-43 (N-terminal) Recombinant antibody

TDP-43 (N-terminal) Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

16A22

Cat no : 80002-1-RR

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43(N-terminal) Recombinant antibody (80002-1-RR)

    WB analysis using 80002-1-RR

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HAP1 cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    WB analysis of HAP1 using 80002-1-RR

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Western Blot (WB) analysis of HeLa cells using TDP-43(N-terminal) Recombinant antibody (80002-1-RR)

    WB analysis of HeLa using 80002-1-RR

    WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HAP1 cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IP experiment of HAP1 using 80002-1-RR

    HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HeLa cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IP experiment of HeLa using 80002-1-RR

    IP result of anti-TDP-43 (N-terminal) (IP:80002-1-RR, 4ug; Detection:80002-1-RR 1:20000) with HeLa cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IHC staining of mouse brain using 80002-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RREC80002 (TDP-43 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43(N-terminal) Recombinant antibody (80002-1-RR)

    IHC staining of mouse brain using 80002-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IF Staining of rat brain using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HAP1 cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IF Staining of HAP1 using 80002-1-RR

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IF Staining of SH-SY5Y using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IF Staining of HeLa using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    IF Staining of HepG2 using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 80002-1-RR (TDP-43 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

  • Flow cytometry (FC) experiment of HeLa cells using TDP-43 (N-terminal) Recombinant antibody (80002-1-RR)

    FC experiment of HeLa using 80002-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 80002-1-RR

    Affinity and Kinetic Characterization of 80002-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80002-1-RR against Human TDP-43 (N-terminal) were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HAP1, K-562 cells, Neuro-2a cells, C6 cells
Positive IP detected inHAP1 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells, SH-SY5Y cells, HAP1 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80002-1-RR targets TDP-43 (N-terminal) in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 (N-terminal) fusion protein Ag1231 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.

Protocols

Product Specific Protocols
WB protocol for TDP-43 (N-terminal) antibody 80002-1-RRDownload protocol
IHC protocol for TDP-43 (N-terminal) antibody 80002-1-RRDownload protocol
IF protocol for TDP-43 (N-terminal) antibody 80002-1-RRDownload protocol
IP protocol for TDP-43 (N-terminal) antibody 80002-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Neural Regen Res

5-Hydroxytryptamine: a potential therapeutic target in amyotrophic lateral sclerosis

Authors - Shi-Shi Jiang
View Article
humanWB,IF,IP

F1000Res

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence

Authors - Donovan Worrall
View Article
humanIHC

J Neuropathol Exp Neurol

COVID-19 could worsen cerebral amyloid angiopathy

Authors - Julie Dewisme
View Article