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  • Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Monoclonal antibody, PBS Only

TDP-43 (C-terminal) Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5F6

Cat no : 67345-1-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43, TDP-43 (C-terminal)

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    WB analysis of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    WB result of TDP-43 (C-terminal) antibody (67345-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 (C-terminal) transfected HeLa cells. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • WB analysis using 67345-1-Ig (same clone as 67345-1-PBS)

    WB analysis using 67345-1-Ig (same clone as 67345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67345-1-Ig (same clone as 67345-1-PBS)

    IHC staining of mouse brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    IHC staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 67345-1-Ig (same clone as 67345-1-PBS)

    IHC staining of human gliomas using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    IF Staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    FC experiment of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human TDP-43 (C-terminal) (67345-1-Ig, Clone:1G5F6) (red) or 0.20 ug Mouse IgG2a Isotype Control(66360-2-Ig, CloneK11A1B2A2) (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67345-1-PBS targets TDP-43 (C-terminal) in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.