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  • Featured Product
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TDRD3 Polyclonal antibody

TDRD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 13359-1-AP

Synonyms

Tudor domain-containing protein 3, tudor domain containing 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TDRD3 Polyclonal antibody (13359-1-AP)

    WB analysis of HEK-293 using 13359-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13359-1-AP (TDRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TDRD3 Polyclonal antibody (13359-1-AP)

    WB analysis of HEK-293 using 13359-1-AP

    WB result of TDRD3 antibody (13359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDRD3 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using TDRD3 Polyclonal antibody (13359-1-AP)

    IP experiment of HeLa using 13359-1-AP

    IP result of anti-TDRD3 (IP:13359-1-AP, 3ug; Detection:13359-1-AP 1:1000) with HeLa cells lysate 3800ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDRD3 Polyclonal antibody (13359-1-AP)

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDRD3 Polyclonal antibody (13359-1-AP)

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDRD3 Polyclonal antibody (13359-1-AP)

    IF Staining of HeLa using 13359-1-AP

    Immunofluorescent analysis of HeLa cells using 13359-1-AP (TDRD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13359-1-AP targets TDRD3 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDRD3 fusion protein Ag4174 相同性解析による交差性が予測される生物種
Full Name tudor domain containing 3
Calculated molecular weight 651 aa, 73 kDa
Observed molecular weight 73 kDa, 83 kDa
GenBank accession numberBC030514
Gene symbol TDRD3
Gene ID (NCBI) 81550
RRIDAB_2202810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

Protocols

Product Specific Protocols
WB protocol for TDRD3 antibody 13359-1-APDownload protocol
IHC protocol for TDRD3 antibody 13359-1-APDownload protocol
IF protocol for TDRD3 antibody 13359-1-APDownload protocol
IP protocol for TDRD3 antibody 13359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Theranostics

A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis.

Authors - Bing-Ling Peng
View Article
humanWB

Int J Biol Macromol

PRMT1 and TDRD3 promote stress granule assembly by rebuilding the protein-RNA interaction network

Authors - Mengtong Qin
View Article