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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TDRD3 Polyclonal antibody

TDRD3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13359

Synonyms

Tudor domain-containing protein 3, tudor domain containing 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TDRD3 Polyclonal ant (CL488-13359)

    IF Staining of HeLa using CL488-13359

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TDRD3 antibody (CL488-13359) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated TDRD3 Polyclonal ant (CL488-13359)

    FC experiment of HEK-293 using CL488-13359

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Tdrd3 Polyclonal Antibody (CL488-13359)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13359 targets TDRD3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDRD3 fusion protein Ag4174 相同性解析による交差性が予測される生物種
Full Name tudor domain containing 3
Calculated molecular weight 651 aa, 73 kDa
Observed molecular weight 73 kDa, 83 kDa
GenBank accession numberBC030514
Gene symbol TDRD3
Gene ID (NCBI) 81550
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TDRD3 antibody CL488-13359Download protocol
FC protocol for CL Plus 488 TDRD3 antibody CL488-13359Download protocol
Standard Protocols
Click here to view our Standard Protocols